4.6. Apomorphine-Induced Rotation

Apomorphine (0.5 mg/kg in 0.1% ascorbic acid, s.c.)-induced contralateral rotations were recorded using a digital video camera to count the number of rotations for 60 min at weekly intervals up to 4 weeks post-lesion (i.e., 7, 14, 21, and 28 days post-lesion) using the same test conditions. The net number of rotations was calculated (contralateral minus ipsilateral full turns). In unilaterally 6-OHDA lesioned animals, apomorphine-induced contralateral rotation is a well-established correlate of dopamine depletion, with rotation levels seen here indicative of an 80–90% loss [53].