DQ-OVA antigen processing assay

MoDCs (5 x 10⁴ cells) in 100 μl of media were incubated with 10 μg/ml DQ-OVA (Thermofisher Scientific) for 1 hour at 4°C or 37°C. Controls consisting of MoDCs without DQ-OVA were included. MoDCs were washed with FACS buffer (PBS containing 1% newborn calf serum (NCS; ThermoScientific) and 1% bovine serum albumin (BSA; Sigma-Aldrich)) and percentages of DQ-OVA⁺ MoDCs analysed by flow cytometry; DQ-OVA is conjugated to BODIPY FL dye and emits fluorescence maximally between 505nm and 515nm.