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Transmission Electron Microscopy

RI Ryoichi Ishibashi
MT Minoru Takemoto
YA Yoshihiro Akimoto
TI Takahiro Ishikawa
PH Peng He
YM Yoshiro Maezawa
KS Kenichi Sakamoto
YT Yuya Tsurutani
SI Shintaro Ide
KI Kana Ide
HK Harukiyo Kawamura
KK Kazuki Kobayashi
HT Hirotake Tokuyama
KT Karl Tryggvason
CB Christer Betsholtz
KY Koutaro Yokote
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Mice were fixed in 4% paraformaldehyde in PBS solution by perfusion fixation. Tissue samples were collected and fixed in phosphate-buffered 2.5% glutaraldehyde (pH 7.4), post osmicated, and dehydrated with graded alcohol. After immersion in propylene oxide, the specimens were embedded in Epon 812. Ultrathin sections were prepared and collected on electron microscopic grids and examined with a transmission electron microscope (JEM-1011; JEOL).

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