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To analyze corticosterone level in the serum of PTSD and non-PTSD rats, approximately 2 ml blood was obtained from the orbital sinus of the rat’s eye (28). Some samples were collected before training, and some after the testing. Consequently, the blood samples were centrifuged under the following settings: 3500 rpm for 5 min at room temperature. The serum was stored at -70 °C. Corticosterone was quantified using an Enzyme-linked immunosorbent assay (ELISA) DRG Corticosterone kit (Cat. No. EIA4164).
Copyright and License information: ©2018 Iranian Journal of Basic Medical Sciences
This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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