Normal liver cell lines HL-7702, HCC, and liver cancer cell lines HepG2 and SMMC-7721 were purchased from the ATCC company (USA). All the cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, USA). The cells were kept in the humidified incubator at 37°C with 5% CO2. Polydatin was purchased from Sigma (USA).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.