The treadmill test was conducted using the ZH‐PT animal running experiment (Zhenghua Biologic Apparatus Facilities) at 15 degrees downhill. Mice were warmed up at 5 m/min for 2 min, and then ran on the treadmill at 7 m/min for 2 min, 8 m/min for 2 min, and 12 m/min for 5 min. The speed was subsequently increased by 1 m/min to a final speed of 20 m/min. Exhaustion was defined as the inability of the animal to remain on the treadmill despite electrical prodding for 10 s. The running time and distance were calculated.
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