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Ion flux measurement using ICP-OE

AF Azita Fazelkhah
KB Katrin Braasch
SA Samaneh Afshar
ES Elham Salimi
MB Michael Butler
GB Greg Bridges
DT Douglas Thomson
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In this work, in order to estimate the required parameters for the quantitative model, the ion fluxes through the channels and pumps were determined using a flux-based assay with a tracer element. Here, Rb+ was used as a tracer of potassium to study the flux through the potassium channels and Na+/K+ ATPase pumps23,24,47. In the Rb+ assay, cells were incubated with a buffer containing Rb+ for 0.5-2 hours. At specific time intervals, cells were washed to remove extracellular Rb+ and channel activity was determined by measuring the rubidium concentration of the cell lysate and supernatant using an ion specific tool, inductively coupled plasma optical emission spectroscopy (ICP-OES, Varian 725-ES, (Agilent, Australia)).

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