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The activity of alanine aminotransferase (ALT) in plasma was determined with the biochemical assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, People's Republic of China). Liver tissue (200 mg) was homogenized in 3 ml of ethanol-acetone mixture (1:1 in volume). The total hepatic TG was extracted in the medium at 4°C, overnight. After being centrifuged at 1,000 g for 20 min at 4°C, the supernatant was removed for the TG assay according to the instruction of the commercial kit (Dongou Bioengineering, Zhejiang, People's Republic of China).
Copyright and License information: ©2018 Peng, Leng, Tian, Yang, Fang, Feng, Zhao and Hu.
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