Purification of polyclonal antibodies from the mouse peritoneal cavity

TK Te Ha Kim
DK Dongbum Kim
AG Avishekh Gautam
HL Heesu Lee
MK Min Hyung Kwak
MP Min Chul Park
SP Sangkyu Park
GW Guang Wu
BL Bok Luel Lee
YL Younghee Lee
HK Hyung-Joo Kwon
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The mice were administered PBS or CpG-DNA 1826 via an i.p injection. Seven days after administration, supernatants from the peritoneal cavity fluid were prepared by centrifugation to remove the peritoneal cells. Polyclonal antibodies were purified from the resulting supernatants using Protein A affinity chromatography (Repligen, Waltham, MA, USA) and analyzed by SDS-PAGE. The ability of these antibodies to bind S. aureus MW2 was measured by ELISA as described above.

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