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Taxonomic identification of FZC3

YL Yuanwang Liu
HC Huiqing Chang
ZL Zhaojun Li
CZ Cheng Zhang
YF Yao Feng
DC Dengmiao Cheng
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The isolated fungus strain that showed the best gentamicin removal was designated as FZC3 and was grown on PDA medium for 5 days at 30 °C. High-molecular-weight DNA was obtained using the CTAB method25. DNA fragments covering partial 18S rRNA, ITS1, 5.8S rRNA, ITS2, and partial 28S rRNA sequences were amplified and sequenced11. The sequences were compared with known sequences in NCBI using the BLAST programs. In addition, FZC3 was also morphologically analyzed16. FZC3 was stored under preservation number CGMCC 12072 under the Genebank accession number of KU170490.

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