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Membrane potential measurements

ES Esin B. Sözer
CP C. Florencia Pocetti
PV P. Thomas Vernier
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Cells were incubated with the membrane-potential-sensitive dye FluoVolt™ (Molecular Probes (Thermo Fisher Scientific)) at 1:500 dilution at room temperature for 30 min before washing and resuspension in RPMI 1640 for pulse exposure. For image analysis, a ring-shaped region enclosing the cell membrane was selected as the region of interest (ROI) for each cell. The change in membrane potential is proportional to the ratio of the change in mean fluorescence to the initial fluorescence (∆F/F0) in the ROI. Sham exposures were recorded with cells treated with the same protocol but without delivery of the pulsed electric field. Sham exposures were subtracted from each data point.

Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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