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Blood collection, RNA purification, and RT-qPCR
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Whole blood samples were collected in PAXgene tubes prior to coronary angiography. PAXgene tubes were processed according to the manufacturer’s instructions, and then frozen at −20 °C. RNA was purified from 400ul PAXgene solution using the magnetic bead-based Agencourt RNAdvance system. PCR primers used for RT-qPCR have been previously described for ITGA2B [9]; primers for the genes in the smoking GES can be found in the Additional file 1: Table S2 RT-qPCR was performed using the Roche Light Cycler 480 system.
Copyright and License information: The Author(s) ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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