The soft 75% ethanol Magnoliae Cortex extract was provided by Dongbang FTL (Seoul, Korea). The titrated, unsaponifiable maize extract fraction was provided by Dongkook Pharmaceutical Co., Ltd. (Seoul, Korea). Ibuprofen (Sigma-Aldrich, St. Louis, MO, USA) was used as a positive control. Dimethyl sulfoxide (DMSO) was used as a solvent. The final soft 75% ethanol Magnoliae Cortex extract concentration was 60 μg/mL in 1% DMSO; this was denoted as M. The final concentration of the titrated unsaponifiable maize extract fraction was 300 μg/mL in 1% DMSO; this was denoted as Z. The combined treatment of M and Z was denoted as MZ. The final ibuprofen concentration was 10 mM in 1% DMSO; this was denoted as IBU.
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