4.7. In situ hybridization analyses

For in situ hybridization, sections were hybridized with antisense or sense ³⁵S-labeled riboprobes as described in detail previously[51]. cDNA clones used as templates for probes included: Sox9 (nt, 116-856; {"type":"entrez-nucleotide","attrs":{"text":"NM_011448","term_id":"165932320","term_text":"NM_011448"}}NM_011448), Tenascin-C (Tn-C) (nt. 151-741; {"type":"entrez-nucleotide","attrs":{"text":"NM_011607","term_id":"313851063","term_text":"NM_011607"}}NM_011607); Collagen II (Col II) (nt.1095-1344; {"type":"entrez-nucleotide","attrs":{"text":"X57982","term_id":"50490","term_text":"X57982"}}X57982), Collagen X (Col X) (nt.1302-1816; NM009925), Indian hedgehog (Ihh) (nt. 897-1954; MN_010544) and Mmp13 (nt. 11-744; {"type":"entrez-nucleotide","attrs":{"text":"BC125320","term_id":"116138811","term_text":"BC125320"}}BC125320).