2.1. Preparation of Nanoemulsion (NE) and Nanosphere (NS) formulations

To prepare NE formulation, an organic phase containing 0.038 g of sorbitan monostearate, 0.160 g of oil (capric/caprylic triglyceride) and 27 mL acetone was injected into an aqueous phase containing 0.080 g polysorbate 80 and 53 mL ultrapure water, under magnetic stirring at 40 °C. To prepare NS, the organic phase was composed by 0.100 g poly(ε-caprolactone), 0.038 g of sorbitan monostearate solubilized in 27 mL acetone. This phase was injected into an aqueous phase containing 0.080 g polysorbate 80 and 53 mL ultrapure water, under magnetic stirring at 40 °C. For both formulations, after 10 min, the organic solvent was removed under reduced pressure at 40 °C using a rotary evaporator (Büchi, Switzerland), having their volume reduced to 10 mL. The formulations prepared in triplicate batches (n = 3).