miRNA mimic and CYP11B2 overexpression plasmid transfection

miR‐193a‐3p mimic and negative control (NC) miRNA mimic were purchased from RiboBio Co. (Guangzhou, China). H295R cells were plated at 50% confluency and transfected with 100 nM miR‐193a‐3p mimic or NC mimic, using Lipofectamine^® RNAiMAX Transfection Reagent (Invitrogen) according to the manufacturer's protocol. Full‐length CYP11B2 ({"type":"entrez-nucleotide","attrs":{"text":"NM_000498","term_id":"119829182","term_text":"NM_000498"}}NM_000498) was cloned into the expression plasmid pcDNA3.0. Transfection of the plasmid was conducted using Lipofectamine^® 2000 (Invitrogen) according to the manufacturer's instructions.