Statistical analysis was performed using SPSS Statistics 20.0 software (IBM, Armonk, NY, USA). All values were expressed as the mean ± SE from three independent experiments. Student’s t test was performed to analyze the differences between two groups, and for multiple comparisons a one-way analysis of variance (ANOVA) followed by Tukey’s test was conducted. P < 0.05 was considered statistically significant.
Copyright and License information: The Author(s). ©2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.