Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Morphologic analysis

LV Laura A. Velazquez-Villegas
AP Alessia Perino
VL Vera Lemos
MZ Marika Zietak
MN Mitsunori Nomura
TP Thijs Willem Hendrik Pols
KS Kristina Schoonjans
request Request a Protocol
ask Ask a question
Favorite

scWAT, epidydimal WAT and BAT samples were fixed in buffered formalin (4%) overnight and embedded in paraffin. About 5 μm thick serial sections were made from paraffin-embedded tissue and then stained with haematoxylin and eosin. A Leica DM500 light microscope (Leica Microsystems, Wetzlar Germany) was used for imaging. Quantification of adipocyte area was performed with Adiposoft automated software. Briefly, all the adipocytes were manually selected using ImageJ’s multipoint selection and were separated by using the Watershed transform plugin. Adipocyte area and number were measured as described68.

Copyright and License information: The Author(s) ©2018
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A