We analyzed samples from 134 adults with liver metastases from primary CRC of which 121 metastases from independent patients successfully underwent molecular analysis (Supplementary Fig. 1). The characteristics of these patients are described in Table 1, Supplementary Data 1, and the Supplementary Methods. We utilized a retrospective clinical cohort study design to identify patients who received uniform treatment for limited (defined as 1–5 lesions involving one or both hepatic lobes), resectable de novo CRC liver metastasis (CRCLM) at two collaborating institutions. We obtained appropriate approval from Institutional Review Boards at each respective cancer center.
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