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To evaluate bacterial loads in the spleen, liver, and mesenteric lymph nodes (mLNs), samples of the different organs were homogenized, serially diluted in sterile PBS, and seeded onto LB, MacConkey, or Salmonella-Shigella agar. Plates were supplemented with kanamycin (50 μg/ml) when mutant strains TTSS-1 or TTSS-2 were tested.
Copyright and License information: ©2018 Schultz, Salazar, Paduro, Pardo-Roa, Pizarro, Salazar-Echegarai, Torres, Riedel, Kalergis, Álvarez-Lobos and Bueno.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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