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TUNEL Assay

YL Yanli Li
YT Yuan Tang
SW Shoujie Wang
JZ Jing Zhou
JZ Jia Zhou
XL Xiao Lu
XB Xiaochun Bai
XW Xiang-Yang Wang
ZC Zhengliang Chen
DZ Daming Zuo
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Formalin-fixed and paraffin-embedded tissue sections of liver were deparaffinized in xylene, then gradually rehydrated in decreasing concentrations of ethanol and distilled water. Subsequently, proteinase K permeabilized sections were subjected to incubation with TdT enzyme and fluorochrome mixture (Promega, Madison, WI, USA) for 1 h at 37°C in the dark. After DAPI (Roche, Mannheim, Germany) staining, the slides were analyzed under a fluorescence microscope.

Copyright and License information:  ©2016 Li, Tang, Wang, Zhou, Zhou, Lu, Bai, Wang, Chen and Zuo.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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