Carrageenan-induced inflammation impediment in rats

The antiinflammatory competence of I. batatas was appraised with the help of carrageenan-induced rat paw edema model [10]. Among seven groups of experimental animals (Sprague-Dawley rats) as described above, Group I-IV were test groups, i.e., IPT-EA, IPT-M, IPR-EA and IPR-M extracts at the dose of 300 mg/kg BW, Group V was positive control ibuprofen at the dose of 10 mg/kg BW, Group VI was carrageenan control and Group VII was saline control, which received 0.1 ml normal saline. The drugs were administered an hour before injection of 150 μl of carrageenan suspension (0.9% w/v in saline) in left hind paw of each rat for the duration of one day. The paw volume was measured at different time periods (1, 3, 6, 12, 24 h) by using Plethysmometer (Ugo Basile 7140, Italy). Results were calculated as.

where PVt is paw volume (ml) at specific time after carrageenan administration while PVc is paw volume (ml) before carrageenan administration.

where EVc is edema volume of control group. EVt is edema volume of treated group.