Construction of a subcutaneous xenograft model of HCC in nude mice

A 6-week-old female BALB/c nude mice were acquired from the animal center of the Cancer Institute of the Chinese Academy of Medical Science. As described previously [20,21], a subcutaneous xenograft model of HCC in nude mice was constructed by injection of SMMC-7721 infected cells. We randomly divided the nude mice into 2 groups: the UBAP2L silence group (shUBAP2L) and the control group (NC), and each group had 6 nude mice. After 13 days, the mice were euthanized. The tumor volume was calculated as follows: volume=length×width²/2.