Mouse Model of Bleomycin-Induced Lung Injury

Mice were anesthetized with a combination of Ketamine (80 mg/kg body weight) and Xylazine (5 mg/kg body weight) and placed on an intubation stand facing upward at an angle of approximately 45° by using an elastic string carefully positioned under the animal’s front incisors. The tongue was gently pulled out with forceps and the trachea was intubated with a 22-G plastic sterile i.v. catheter. Bleomycin sulfate (available as 15U Bleocip injection, Cipla) at 100 mU, prepared in 30 μl of sterile saline was then slowly instilled through the catheter into the trachea.