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Cells (2 × 105) were treated with 50 μmol/L of cytarabine (Pharmacia & Upjohn, Kalamazoo, MI, USA), or 50 μmol/L normal saline as the negtive control (NC). Cells were harvested at 24 hours after treatment. Apoptosis was detected using an FITC Annexin V/PI Kit (BD, San Jose, CA, USA), and cells were analyzed immediately by flow cytometry (FACS Aria II; BD). Annexin V+/PI− cells identified early apoptosis.
Copyright and License information: ©2018 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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