Cell viability assay

Cell viability after PLIN protein manipulation was assessed using the MTS CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega) according to the manufacturer’s instructions. Briefly, PLIN2-overexpressing trophoblasts were exposed to palmitic acid or arachidonic acid for 24 hours. MTS substrate was added at a ratio of 20 mL of MTS for every 100 mL of culture medium. After 1.5 hours of incubation, 50 µL medium from each well was analyzed in triplicate on a colorimetric plate reader (Molecular Devices, Sunnyvale, CA) at 490 nm.