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Caspase 3/7 activity assay

LV Laura Vera-Ramirez
SV Suman K. Vodnala
RN Ryan Nini
KH Kent W. Hunter
JG Jeffrey E. Green
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Caspase 3/7 activity was performed using the Caspase-Glo 3/7 Assay (Promega) according to the manufacturer’s protocol. Cells were seeded in 3D cultures as described above with or without 20 μM MitoTEMPO for 5 days. Cells were treated with HCQ at day 5 and assayed after 24 h. Briefly, plates and reconstituted caspase 3/7 reagent were allowed to equilibrate to room temperature (RT), reagent was then added to wells at a 1:1 ratio and incubated for 1 h at RT. Luminescence was measured using aGlomax (Promega) microplate luminometer. Wells with all assay components, except the cells, served as blank controls.

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