Rotarod test was carried out to see the motor coordination of mice, using an Ugo Basile Rota-Rod 47600 (Ugo Basile, Comerio, Italy). The apparatus was programmed to accelerate its rod rotation from 4 to 40 rpm during 300 s. Time from when mouse was put onto the rod of the apparatus until when the mouse fell off or cling on to the rod was recorded. Mice were subjected to three trials per day between 2:00 and 7:00 p.m., and the test was performed for the third straight day.
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