Faecal water content and colonic faecal content

The wet weight of faecal pellets was measured following 14 days in vivo oxaliplatin administration. Individual mice were placed in holding cages for a period of 15–30 min on three separate occasions throughout the day; the first five pellets expelled from every mouse at each holding time were collected and weighed immediately to determine wet pellet weight. Pellets were then dehydrated for 72 h at room temperature prior to measurement of the dry weight. Water content was calculated as the difference between the wet weight and dry weight.