2.5. Heterologous whole cell competition binding assay

Competition binding was performed as previously described [41] on whole cells in 96-well plates by using the antagonist radioligand ¹²⁵I-sCT(8-32) (∼0.1 nM), and competing with increasing concentrations of unlabelled peptide. Non-specific binding was defined by co-incubation with 1 μM unlabelled sCT(8-32). Following overnight incubation non-bound ligand was removed and radioactivity was determined using a gamma counter.