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Hemolysis test

CL Chunxi Liu
TL Tingxian Liu
YL Yongjun Liu
NZ Na Zhang
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Hemolysis test was carried out to investigate the safety of CPN-PDR for intravenous injection. A 2% red blood cell suspension was collected by centrifugation and resuspension. The test was performed under the design described in Table 1. After incubated at 37°C for 3 h, all the groups were centrifuged at 3,000 rpm for 15 min and visualized by the naked eye. To measure the hemolysis ratios of each group, UV-vis spectrophotometry was carried out to record the absorbance of supernatant in each group. The quantitation of hemolysis ratios was calculated according to the following formula:

where Abs (sample), Abs (−) and Abs (+) refer to the absorbances of the samples, negative control and positive control, respectively.

Design of hemolysis test

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