Compatibility of protease with commercial detergents

Compatibility of protease with commercial detergents (Axion^®, Surf Excel^®, Ariel^®, Dixan^®, Sunlight^® and Top^®) were assessed. Solid detergents were diluted in distilled water (0.7%, w/v), whereas liquid detergents were diluted 100-fold to stimulate washing condition (Niyonzima and More 2015b). Diluted detergent solutions were boiled for 15 min to denature the potential enzymes originally presented, if any, and confirmed by protease assay. Detergent solutions were incubated with crude protease from strain CD6 at 50 °C for 1 h and then assayed by standard protease assay. Relative activity (%) was expressed by taking enzyme activity without any commercial detergent (control) as 100%.