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Sample collection and DNA preparation

PC Pengyu Chen
GH Guanglin He
XZ Xing Zou
XZ Xin Zhang
JL Jida Li
ZW Zhisong Wang
HG Hongyan Gao
LL Li Luo
ZZ Zhongqing Zhang
JY Jian Yu
YH Yanyan Han
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Peripheral blood samples were collected from 308 unrelated healthy Chinese individuals from three Chinese major population groups in Guizhou Province, southwest China, including: 98 Bouyei individuals residing in Qiannan District, 101 Han individuals recruited from Zunyi District, 109 Miao individuals residing in Qiandongnan District. One milliliter of blood was obtained in tubes with EDTA. The ancestors of all subjects must live in the present region at least three generations. The QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) was used to extract the genomic DNA based on the manufacturer’s recommendations. A 7500 Real-time PCR system (Thermo Fisher Scientific) was employed to determine the DNA concentration using Quantifiler Human DNA Quantification Kit. The DNA was diluted to 1 ng/μL and stored at −20 °C until amplification.

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