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Yeast two-hybrid assay (Y2H) was performed following the Clontech yeast protocol handbook using the small-scale lithium acetate yeast transformation method53. In brief, yeast cells (strain AH109) were plated onto a selective medium lacking tryptophan and leucine (SD-Trp-Leu) to confirm the right transformation and a high-stringency selective medium lacking tryptophan, leucine, histidine, and adenine (SD-Trp-Leu-His-Ade) to detect positive interactions.
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