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Two-electrode voltage clamp

WZ Wang Zheng
XY Xiaoyong Yang
RH Ruikun Hu
RC Ruiqi Cai
LH Laura Hofmann
ZW Zhifei Wang
QH Qiaolin Hu
XL Xiong Liu
DB David Bulkley
YY Yong Yu
JT Jingfeng Tang
VF Veit Flockerzi
YC Ying Cao
EC Erhu Cao
XC Xing-Zhen Chen
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Two-electrode voltage clamp experiments using oocytes were carried out as previous61. Briefly, two electrodes made of capillary pipettes (Warner Instruments, Hamden, CT) penetrating an oocyte were filled with 3 M KCl to obtain a tip resistance ranging from 0.3 to 2 MΩ. Whole-cell currents of an oocyte were recorded at RT in an extracellular solution with composition specified in figure legends. Electric currents were recorded with a Geneclamp 500B amplifier and Digidata 1322 A AD/DA converter (Molecular Devices, Union City, CA). Currents and voltage were recorded at 200 μs/sample and Bessel filtered at 2 kHz. The data was acquainted and analyzed with software pClamp version 9 (Axon Instruments, Union City, CA). SigmaPlot version 13 (Systat Software, San Jose, CA) and GraphPad Prism version 7 (GraphPad Software, San Diego, CA) were employed for data plotting.

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