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Following the treatments of the paired cells as described above, nuclear proteins at each of the time points (4, 24, 48 h) were extracted using the Active Motif kit as per the manufacturer’s instructions. The extracted nuclear proteins (10 μg/condition) were tested for NF-κB p65 transcription factor activity using the TransAM NF-κB p65 kit following the manufacturer’s instructions. The data were expressed as optical density (OD) value.
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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