2.3. SiRNA transfection

ON-TARGETplus siRNA was purchased from Dharmacon (GE Healthcare Life Sciences, CO, USA) for two individual PARG ({"type":"entrez-nucleotide","attrs":{"text":"NM_003631","term_id":"1198333560","term_text":"NM_003631"}}NM_003631) siRNA oligonucleotides, PARP1 ({"type":"entrez-nucleotide","attrs":{"text":"NM_001618","term_id":"1519246470","term_text":"NM_001618"}}NM_001618) and the non-targeting siRNA #1 (scramble) control. All siRNAs were resuspended at 20 μM in 1 × siRNA universal buffer (Dharmacon) and stored at −20 °C. Cells were seeded in 6-well plates and left overnight to attach. The following day, cells were transfected with 20 nM siRNA (final concentration) using Dharmafect 4 reagent (Dharmacon) following manufacturers’ instructions. Knockdown was confirmed after 48 h by western blotting.