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Cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum, 1 mM sodium pyruvate, 1% MEM non-essential amino acids, 2 mM l-glutamine, 100 µg/ml streptomycin, 100 U/ml penicillin, 10 µg/ml gentamicin, 55 µM 2-mercaptoethanol, and 10 mM HEPES buffer (complete RPMI). Isolated human mast cells were cultured in the presence of human SCF (20 ng/ml, Shenandoah Biotechnology). Mast cell phenotypes were assessed by flow cytometry (c-Kit+FcεRIα+) and chloroacetate esterase staining.
Copyright and License information: ©2018 Burton, Epp, Fanny, Miller, Stranks, Teague, Clark, van de Rijn and Oettgen.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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