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Cells were fixed, washed with PBS, treated with RNaseA, and stained (37°C, 30 min) with 25 µg/ml propidium iodide (PI). The samples were then analyzed via flow cytometry, and the cell cycle phase distribution was quantified using Modfit Software (BD Biosciences, Franklin Lakes, NJ, USA). The proliferative index was calculated to represent the % of cells identified to occur in the S/G2/M phase.
Copyright and License information: ©2018 Han et al.
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