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Lysosome imaging in live cells
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U-2 OS cells were treated with 5 μM Morph-Az, in culture media for 1 h at 37 °C. After incubation, cells were washed and added with 2 μM CO-1 probe then proceed for 1 h incubation at 37 °C. Following incubation, cells were treated with nuclei dye Hoechst 33342 (1 μg μl−1) and LysoTracker Red (1 μM). After counterstaining, cells were washed three times with growth media and imaged using Nikon A1R+ confocal laser microscope system.
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