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Induction of A549 xenografts in nude mice
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Tumors were induced in female nude mice (NMRI-nu/nu) by subcutaneous inoculation of 1 × 107 A549 cells into the the right dorsal flank. Animals were injected intraperitoneally every 72 h with test substances or its vehicles for 28 days. The treatment was started 7 days after tumor induction. Tumor volume was calculated as (4π/3) x (width/2)2 x (length/2). Experiments were conducted in accordance with the policies of local Animal Ethics Committee.
Copyright and License information: ©2016 Winkler et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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