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Mice models of liver fibrosis

PH Pushpa Hegde
EW Emmanuel Weiss
VP Valérie Paradis
JW Jinghong Wan
MM Morgane Mabire
SS Sukriti Sukriti
PR Pierre-Emmanuel Rautou
MA Miguel Albuquerque
OP Olivia Picq
AG Abhishak Chandra Gupta
GF Gladys Ferrere
HG Hélène Gilgenkrantz
BK Badr Kiaf
AT Amine Toubal
LB Lucie Beaudoin
PL Philippe Lettéron
RM Richard Moreau
AL Agnès Lehuen
SL Sophie Lotersztajn
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Animals were housed in pathogen-free animal facility and fed ad libitum. Liver fibrosis was induced in male mice (12–14-week old) by either repeated injections of carbon tetrachloride (CCl4, 0.5 ml/kg body weight, 1:10 dilution in mineral oil, Sigma, France), twice a week for 4 weeks (Vα19 TCR transgenic, n = 9; WT littermates n = 10 obtained from two independent experiments (exp 1, n = 5 WT, n = 4 Tg; exp 2, n = 5 WT, n = 5 Tg); MR1−/−, n = 5; WT littermates n = 6), or bile duct ligation and section (Vα19 TCR transgenic, n = 9; WT littermates n = 3), as we previously described47,48. Animals were sacrificed 24 h after the last CCl4 injection or 12 days after surgery in BDL mice. Experiments were performed in accordance with protocols approved by the Paris-Nord ethical committee C2EA 121 (authorization number 02529.02).

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