2.9. Drugs and reagents

All chemicals used in electrophysiology studies were purchased from Sigma-Aldrich unless otherwise specified. Ganaxolone was prepared as 2 mM stock solutions in dimethyl sulfoxide for electrophysiology experiments. Stock solutions were diluted in the external perfusion solution to the desired concentration for electrophysiological use. The concentration of dimethyl sulfoxide in final solution was less than 1%. GX and kynurenic acid were acquired from Tocris. TTX was purchased from Calbiochem (Billerica, MA).