2.6. Determination of Minimum Bactericidal Concentration (MBC)

The MBC is the lowest concentration of antibiotic agent that kills at least 99.9% of the organisms. To determine MBC using Doughari modified method [9], 0.5 mL of the sample was removed from those tubes from MIC which did not show any visible sign of growth and inoculated on sterile Mueller Hinton agar by streaking. The plates were then incubated at 37 °C for 24 h. The concentration at which no visible growth was seen was recorded as the minimum bactericidal concentration (Table 4).