Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Transformation of plasmids into S. cerevisiae

KM Katherine A. Murphy
CT Christine A. Tabuloc
KC Kevin R. Cervantes
JC Joanna C. Chiu
request Request a Protocol
ask Ask a question
Favorite

DNA vector p406TEF1 containing inverted repeats of D. suzukii target gene sequence were transformed into S. cerevisiae strain INVSc1 (Life Technologies, Grand Island, NY) using the Frozen EZ Yeast Transformation kit II (Zymo, Irvine, CA) following manufacturer’s instructions. Transformants were selected on minimal media without uracil and confirmed by colony PCR. PCR reactions were performed as described above with a forward primer located upstream of the multiple cloning site and the reverse primer located within the white intron.

Copyright and License information:  ©2016, Macmillan Publishers Limited
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A