HUDEP-2 cell culture

HUDEP-2 cells were the kind gift of Dr. Yukio Nakamura (Kurita et al. 2013). HUDEP-2 cells were expanded in StemSpan SFEM medium (Stem Cell Technologies) supplemented with 50 ng/mL human stem cell factor (SCF) (Peprotech), 3 IU/mL erythropoietin (Amgen), 1 µg/mL doxycycline (Sigma), 1 µM dexamethasone (Cayman), and 100 U/mL penicillin/streptomycin. To induce erythroid differentiation, HUDEP-2 cells were cultured in Iscove's modified Dulbecco's medium (IMDM) (Life Technologies) supplemented with 5% human AB serum (Sigma), 50 ng/mL SCF, 3 IU/mL erythropoietin (Amgen), 1 µg/mL doxycycline (Sigma), 330 µg/mL holo-transferrin (Sigma), 10 µg/mL human insulin (Sigma), 2 IU/mL heparin (Sigma), and 100 U/mL penicillin/streptomycin.