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A549 cells and PC-9 cells were seeded in 96-well plates and cultured overnight of adhesion, then treated with different concentrations of honokiol (0, 20, 40, 60, and 80 μM) for 24, 48, and 72 h, respectively; 10 μl of MTT solution (5 mg/ml) was added in each well and incubated for another 4 h. Then 100 μl of DMSO was added in each well and incubated for 4 h to dissolve the formazan crystals. Absorbance of plates was measured at 570 nm with Tecan microplate reader (Morrisville, NC, United States).
Copyright and License information: ©2018 Dai, Li, Jiang, Wei, Luo, Yao, Li and Leung.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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