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Progranulin levels in brain samples were determined by ELISA (Adipogen) using the manufacturer’s protocol as previously described (Arrant et al., 2015). Brain samples were lysed in a mild NP-40 lysis buffer (10 mM Tris, 10 mM NaCl, 3 mM MgCl2–6H2O, 1 mM EDTA, 0.05% NP-40) and diluted 1:1 with ELISA buffer before analysis.
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