Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Redox Status and Nuclear Factor Erythroid 2-Related Factor (Nrf2) Activated Enzymes Activities

GC Gina Cavaliere
EV Emanuela Viggiano
GT Giovanna Trinchese
CF Chiara De Filippo
AM Antonietta Messina
VM Vincenzo Monda
AV Anna Valenzano
RC Raffaele I. Cincione
CZ Christian Zammit
FC Fabiano Cimmino
AC Angela Catapano
FS Francesco Sessa
GM Giovanni Messina
MM Marcellino Monda
MC Marianna Crispino
MM Maria Pina Mollica
request Request a Protocol
ask Ask a question
Favorite

Reduced glutathione (γ-L-glutamyl-L-cysteinyl-glycine, GSH) and oxidized glutathione (γ-glutamyl-L-cysteinyl glycine disulfide, GSSG) concentrations in the hypothalamus were measured using the dithionitrobenzoic acid (DTNB) GSSG reductase recycling assay (Bergamo et al., 2007). The GSH/GSSG ratio was used as an oxidative stress marker. The enzymatic activities of glutathione S-transferase (GST) and NAD(P)H–quinone oxidoreductase (NQO1) were evaluated spectrophotometrically in cytoplasmic extracts with standard protocols (Benson et al., 1980; Levine et al., 1990).

Copyright and License information:  ©2018 Cavaliere, Viggiano, Trinchese, De Filippo, Messina, Monda, Valenzano, Cincione, Zammit, Cimmino, Catapano, Sessa, Messina, Monda, Crispino and Mollica.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A