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Identification of ribosome binding sites (RBS)
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For identification of RBSs, all 5´-UTRs with a minimal length of 20 nt were analyzed. First, the frequencies of purines (G and A) were compared with the frequencies of pyrimidines (T and C) for every nucleotide position within the 20 nt long sequence upstream of the translation start codon. Sequences with an accumulation of purines (>55%) were extracted. The extracted sequences were used to search for a conserved RBS motif with Improbizer [46]. Resulting data were visualized with Origin (OriginLab) and WebLogo [51].
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